Table 1.
Oligonucleotide primers used in amplifying Cα1 and Cs cDNAs
| Primer | Description | 5′ to 3′ nucleotide sequence |
|---|---|---|
| Cαa | Bovine Cα1 7–26 | AACGCCGCCGCCGCCAAGAA |
| Cαb | Bovine Cα1 328–347 | TCCTTCAAGGACAACTCAAA |
| CαcR | Complement of bovine Cα1 782–800 | TTCAAGTCAGAGCTGAAGT |
| CαdR | Complement of bovine Cα1 928–947 | ATGAAGGGAGCTTCCACCTT |
| CαeR | Complement of bovine Cα1 929–955 | ACTTTGGTATGAAGGGAGCTTCCACCT |
| oCα376 | Ovine Cα1 376–402 (equivalent to ovine Cs 352–378) | GGTGGGGAGATGTTCTCACACCTGCGA |
| oCα482R | Complement of ovine Cα1 456–482 (equivalent to ovine Cs 432–458) | AGCGAGTGCAGGTACTCTAAGGTCAGG |
| oCs(−11) | Ovine Cs −11 to 16 | AAGACTGAGTGATGGCTTCCAACCCCA |
| mCα791R | Complement of murine Cα1 771–791 | GAGCTGAAGTGGGATGGGAAC |
| mCs(−188) | Murine Cs −188 to −167 | GTTCTATCTGCCCCTACCCTGC |
| hCα(−60) | Human Cα1 −60 to −43 | GCCGCAGCCAGCACCCGC |
| AP1 | Adaptor primer | CCATCCTAATACGACTCACTATAGGGC |
| Nested AP1 | Adaptor primer | ACTCACTATAGGGCTCGAGCGGC |
Numbers represent nucleotide positions in Cα1 or Cs mRNA. Nucleotides upstream of a translation start site are numbered 3′ to 5′ beginning with −1; those downstream are numbered 5′ to 3′ beginning with +1.