Table 3.

Generation of ovine, murine, and human C_s clones by 5′-RACE

	First round (5′-RACE)		Second round
	Primers	Thermocycler conditions	Primers	Thermocycler conditions
Clone 6 (ovine)	AP1, CαeR	Annealing at 59°C, extension at 68°C, 40 cycles, final 10-min extension at 68°C	–	–
Clone 7 (murine)	AP1, mCα791R	Annealing at 56°C, extension at 68°C, 40 cycles, final 10-min extension at 68°C	Nested AP1, oCα482R	Annealing at 59°C, extension at 68°C, 35 cycles, final 10-min extension at 68°C
Clone 8 (human)	AP1, CαeR	Annealing at 59°C, extension at 68°C, 40 cycles, final 10-min extension at 68°C