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The complete 3′UTR (−) of DEN4 was synthesized, and two shorter RNA probes were generated. The secondary structures for the region between nt 101 and 1 of DEN4 3′UTR (−) RNA and the shorter RNA probes, nt 101 to 44 and nt 44 to 1, were predicted using the mfold2 program (http://mfold2.wustl.edu). The numbers correspond to the 5′UTR of the positive-strand RNA.
