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. 2003 Mar;77(5):3067–3076. doi: 10.1128/JVI.77.5.3067-3076.2003

FIG. 3.

FIG. 3.

Mobility shift assay of the complete 3′UTR (−) and shorter RNA probes of DEN4. (A) Labeled RNA consisting of nt 101 to 1 was incubated without (lane 1) or with 3 μg of S10 extract from infected U937 cells (lanes 2 to 5), in the absence (lane 2) or in the presence of a 25-fold molar excess of unlabeled 3′UTR (−) RNA (lane 3) or a 25-fold molar excess of the 3′UTR of the DEN4 genome (lane 4) or a 25-fold excess of the complete 5′UTR of the DEN4 genome (lane 5). (B) Labeled RNAs consisting of nt 101 to 1 (lane 1), nt 101 to 45 (lane 2), and nt 44 to 1 (lane 3) were incubated with 3 μg of S10 extract from infected U937 cells. Arrows indicate the migration of the complexes.