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. 2003 Mar;77(5):3067–3076. doi: 10.1128/JVI.77.5.3067-3076.2003

FIG. 7.

FIG. 7.

Mobility shift and UV-induced cross-linking assay of the 3′UTR (−) and deleted RNA probes of DEN4. (A) Labeled RNA consisting of nt 101 to 1 (lanes 1 and 2), del-62 (lane 3), or del-96 (lane 4) was incubated without (lane 1) or with (lanes 2 to 4) 3 μg of S10 extract from infected U937 cells. Complex was assayed by electrophoresis through a 6% native polyacrylamide gel. (B) Labeled 3′UTR (−) RNA consisting of nt 101 to 1 (lane 1), del-62 (lane 2), or del-96 (lane 3) was UV cross-linked with protein present in 50 μg of infected U937 cell extracts. Proteins were separated by SDS-10% PAGE and detected by autoradiography. (C) Labeled 3′UTR (−) RNA consisting of nt 101 to 1 (lanes 1 and 6), del-62 (lanes 4 and 9), del-96 (lanes 5 and 10), or RNA consisting of nt 101 to 45 (lanes 2 and 7) or nt 44 to 1 (lanes 3 and 8) was UV cross-linked with 100 ng of recombinant GST-La protein (lanes 1 to 5) or with GST recombinant protein (lanes 6 to 10). Proteins were separated by SDS-10% PAGE and detected by autoradiography. Molecular masses in kilodaltons are indicated on the left.