FIG. 2.

Cell surface expression of wild-type and mutant LCMV GPs. (A) HeLa cells were mock treated (control) or transfected with pHCMV expression plasmids encoding wild-type and mutant LCMV GPs, as indicated in Table 1. At 48 h after transfection, cells were analyzed by immunostaining. Cell surface LCMV GP was analyzed by immunostaining with the anti-LCMV GP-directed monoclonal antibody KL25. (B) 293T cells were mock treated (shaded) or transfected with pHCMV expression plasmids for LCMV GP (open). At 48 h after transfection, LCMV GP was detected by flow cytometry with antibody KL25.