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. 2000 Sep;11(9):3045–3060. doi: 10.1091/mbc.11.9.3045

Figure 2.

Figure 2

Syntaxin 11 is localized at the plasma membrane in polarized but not in nonpolarized MDCK cells. A mixed population of MDCK cells stably expressing human syntaxin 11 was grown either for 3 d on polycarbonate filters (A–D) or for 2 h on glass coverslips (E). The cells were stained with antibodies against syntaxin 11 (A, C, and E, green), the lateral plasma membrane protein E-cadherin (B and D), or the late endosomal/lysosomal protein LAMP-2 (E, red). B and D also show nuclear DNA staining with propidium iodide. A and B show horizontal confocal optical sections just above the nuclei. C and D show vertical optical sections with the apical plasma membrane at the top. Syntaxin 11 colocalizes with E-cadherin at the lateral plasma membrane. E shows a representative horizontal optical section through the middle of the cells. Syntaxin 11 does not significantly colocalize with LAMP-2. The absence of a syntaxin 11 signal in neighboring nonexpressing cells (A and E) demonstrates the specificity of the antibody. Bars, 5 μm.