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Endogenous syntaxin 3 localizes to VACs in Caco-2 cells. The human colon carcinoma cell line Caco-2 was grown under low-calcium conditions as described in Figure 4. Endogenously expressed syntaxin 3 (A) and the microvillar protein villin (B) were labeled with the appropriate antibodies. Syntaxin 3 is strongly enriched in VACs that are also positive for villin, indicating that VACs are lined by microvilli (arrows). Bar, 5 μm.
