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. 2003 Mar;77(5):3217–3228. doi: 10.1128/JVI.77.5.3217-3228.2003

FIG. 4.

FIG. 4.

Excision of the BAC vector sequences from enhancerless MCMV genomes. PCRs were performed using as templates genomic DNAs isolated from viral stocks of MCMV (lanes 1 and 6), MCMVdE (lanes 2 and 7), MCMVdE::Luc (lanes 3 and 8), and MCMVdE::Luc-rev (lanes 4 and 9) or from an early MCMVdE::Luc passage (lanes 5 and 10). Two different primer sets were used to examine the correct excision of the BAC vector sequences within the viral genomes. The first primer set contains a primer that anneals with the BAC sequence and a primer located with the EcoRI g fragment (lanes 1 to 5), and the second primer set contains two primers that bind to viral sequences flanking the excision site (lanes 6 to 10). The amplified products were separated on a 1% agarose gel and visualized by ethidium bromide staining. Shown are the products obtained in the PCR. The positions of the size markers are shown at the left. The sizes of the amplified products are indicated by arrows.