TABLE 1.
Genotypes and phenotypes of parental and reassortant viruses used in this study
| Virus | Parental origin of indicated genome segment (protein)a
|
Trypsin cleavage of VP4 (EOP)b | Neutralization by α-VP4 MAb 2G4c | Virion stability during purification (%)d | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 (VP1) | 2 (VP2) | 3 (VP3) | 4 (VP4) | 5 (NSP1) | 6 (VP6) | 7 (NSP3) | 8 (NSP2) | 9 (VP7) | 10 (NSP4) | 11 (NSP5) | ||||
| SA11-4F | F | F | F | F | F | F | F | F | F | F | F | 2.7 × 10−3 | Yes | 93.3 |
| SA11-C13 | S | S | S | S | S | S | S | S | S | S | S | <6.3 × 10−6 | Yes | 10.6 |
| B223 | B | B | B | B | B | B | B | B | B | B | B | <1.2 × 10−5 | No | 0.5 |
| R-A29 | S | S | S | F | S | S | S | S | S | S | S | 1.4 × 10−2 | Yes | 60.6 |
| R-924 | F | F | F | S | F | F | F | F | F | F | F | NDf | Yes | 6.9 |
| R-197 | B | B | B | F | B | B | B | B | B | B | B | <8.0 × 10−6e | No | 8.2 |
| R-202 | F | F | F | F | F | B | F | F | F | F | F | ND | ND | 65.5 |
| R-600 | B | B | B | B | B | F | B | B | B | B | B | ND | ND | 1.2 |
| R-004 | F | F | F | B | F | F | F | F | F | F | F | ND | Yes | 0.5 |
a
Origin of segments: B, B223; F, SA11-4F; S, SA11-C13. Bold type indicates differing segment.
b
EOP, titer in the absence of trypsin/titer in the presence of trypsin (from reference 2).
c
Neutralization phenotype using VP4-specific MAB 2G4 (from reference 3).
d
Percent initial titer of virus grown in the presence of trypsin recovered by a standard CsCl purification procedure (from reference 4).
e
Unexpected phenotypic expression is shown in bold.
f
ND, not determined.