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A monoclonal antibody to the basic region recognizes a variable epitope. The monoclonal antibody 9A11 recognizes only peptides (top panel) or Tat proteins (middle panel) containing the 57RPPQ60 sequence (present in IIIB and not present in 89.6). The amino terminus antibody TR1 recognizes an epitope conserved in both proteins (bottom panel). O.D., optical density. Error bars indicate standard deviations.
