TABLE 1.
Polyclonal sera from macaques immunized with unmodified (Tat) or carboxymethylated Tat (Tat Tx) recognize epitopes that include the amino acid sequence 57RPPQ60
| Peptide | Reaction (optical density)a of serum from animal:
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 95011 (Tat Tx) | 96032 (Tat Tx) | 95042 (Tat Tx) | 96058 (Tat Tx) | 96061 (Tat Tx) | 96079 (Tat) | 96116 (Tat) | 96122 (Tat) | 96134 (Tat) | |
| aa 46-65 (SYGSKKRRQRRRPPQDNQTH) | 2.412, 2.452 | 2.474, 2.457 | 1.558, 1.624 | 1.25, 1.267 | 0.254, 0.257 | 0.053, 0.052 | 1.322, 1.336 | 0.234, 0.259 | 2.025, 2.007 |
| aa 46-60 (SYGSKKRRQRRRPPQ) | 0.867, 0.865 | 1.678, 1.664 | 1.169, 1.098 | 1.142, 1.138 | 0.331, 0.317 | 0.196, 0.182 | 1.052, 1.012 | 0.723, 0.743 | 2.279, 2.318 |
| aa 41-56 (KALGISYGSKKRRQRR) | 0.059, 0.062 | 0.519, 0.533 | 0.145, 0.145 | 0.79, 0.796 | 0.124, 0.105 | 0.036, 0.032 | 0.78, 0.76 | 0.699, 0.648 | 0.071, 0.145 |
a
Results of two determinations are shown.