TABLE 2.
Tunicamycin treatment of hamster cells enables their infection by RD114 and simian type D retroviruses
| Cells | Titer of LacZ pseudotype (CFU/ml)a
|
|||||
|---|---|---|---|---|---|---|
| RD114
|
SRV-2
|
BaEV
|
||||
| (−)Tunic | (+)Tunic | (−)Tunic | (+)Tunic | (−)Tunic | (+)Tunic | |
| CHO | 0 | 1.0 × 105 | 0 | 2.2 × 104 | 4.1 | 5.2 × 104 |
a
CHO cells were tested for their susceptibility to LacZ(RD114), LacZ(SRV-2), and LacZ(BaEV) pseudotype viruses before treatment with tunicamycin [(−)Tunic] and after treatment of the cells with 250 ng of tunicamycin/ml [(+)Tunic] for 24 h. The viruses used in these assays had titers of approximately 105 CFU/ml when examined in human TE671 cells.