TABLE 1.
Effects of sCD4-17b against genetically diverse HIV-1 isolates
| Expta | Isolate | Cladeb | Phenotype (coreceptor usage)b,c | % Neutralization by sCD4-17b at:
|
|
|---|---|---|---|---|---|
| 6.4 nM | 32 nM | ||||
| 1 | Ba-L | B | NSI (R5) | 94 | 98 |
| LAV | B | SI (X4) | 50 | 75 | |
| 2 | 92RW023 | A | NSI (R5) | 0 | 0 |
| Ba-L | B | NSI (R5) | 26 | 99 | |
| 91US054 | B | ns | 0 | 0 | |
| 92US714 | B | NSI (R5) | 0 | 80 | |
| 92US076 | B | SI (R5X4) | 11 | 84 | |
| 93IN905 | C | NSI (R5) | 7 | 6 | |
| 92UG024 | D | SI (X4) | 0 | 65 | |
| 93TH073 | E | NSI (R5) | 0 | 0 | |
| 93BR029 | F | NSI (R5) | 14 | 0 | |
In experiment 1, viruses were preincubated with the indicated concentrations of sCD4-17b in medium and assayed for infectivity, by using MAGI-CCR5 cells for Ba-L and MAGI cells for LAV. The numbers of blue cells per well in untreated controls were 350 for Ba-L and 300 for LAV. In experiment 2, viruses were preincubated with the indicated concentrations of affinity chromatography-enriched sCD4-17b and assayed for infectivity by using MAGI-CCR5 cells. For the different viruses, the numbers of blue cells per well in the untreated controls ranged from 50 to 475.
Clade and coreceptor usage phenotypes are as specified in the AIDS Research and Reference Reagent Program catalogue.
ns, not specified.