FIG. 2.

Kinetics of MHV68 viral protein expression. BHK-21 cells were mock infected (M) or infected with MHV68 in the absence (−) or presence of cycloheximide (c). At various times postinfection (p.i.), as shown at the top of the panels, cell lysates were analyzed by Western blotting with rabbit polyclonal antibody against ORF 45 (A), M9 (ORF 65) (B), or multiple MHV68 lytic proteins (C). Actin was probed, providing a loading control (D).