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Effects of cytosol on BFA-induced Golgi tubule formation in semi-intact cells. Golgi tubule formation was observed in the presence of NEM cytosol/BFA/ATP (A) or BFA/ATP (B) in semi-intact CHO-GT cells. In both cases, the efficiency of the formation was almost the same. However, the motility of the tubules and the frequency of their bifurcation appeared to be increased slightly in the presence of NEM cytosol. Bar, 10 μm.
