Figure 7.

Effect of β-COP depletion on Golgi tubule formation and fusion. Either mock [mock]- or β-COP–depleted [cyto (−β-COP)] rat cytosol containing GTPγS and ATP-regenerating system were subjected to the Golgi tubule formation assay (A) or the Golgi tubule fusion assay (B). The extent of β-COP depletion was determined by immunoblotting (C). When the semi-intact cells were incubated with mock cytosol, GTPγS, and ATP in the Golgi tubule fusion assay, β-COP was found to associate with preformed Golgi tubules as shown in D. Bar, 10 μm.