FIG. 4.

Immunoblot analysis of BYV L-Pro with anti-L-Pro serum. RRL, non-isotope-labeled products of the translation in the rabbit reticulocyte lysates. Triton X-100, fractions that were treated with 2% Triton X-100. Lanes: −RNA, no exogeneous RNA added; Luc, translation of luciferase mRNA; L-Pro, translation of BYV L-Pro mRNA; NI and INF, total protein extracts from the noninfected and BYV-infected plants, respectively; S30 and P30, supernatant and pellet, respectively, after extract centrifugation at 30,000 × g; Virions, isolated BYV virions.