FIG. 2.

Detection of HCV proteins in cell clones supporting H77 subgenomic replication. Two days postseeding, monolayers of the cell clones H-1, H-2, and H-3 and parental Huh-7.5 cells (Mock) were incubated for 10 h in the presence of [³⁵S]methionine and [³⁵S]cysteine. The labeled cells were lysed and immunopreciptated with HCV-positive human serum (JHF, anti-NS3, NS4B, and NS5A), and the labeled proteins were separated by SDS- 10% PAGE. The mobilities of molecular-mass standards are indicated on the left, and the migrations of NS3, NS4B, and NS5A are shown on the right.