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. 2003 Mar;77(5):2998–3006. doi: 10.1128/JVI.77.5.2998-3006.2003

FIG. 4.

FIG. 4.

Vγ2Vδ2+ PBL from SIVmac-BCG-coinfected rhesus monkeys exhibited a reduced capacity to proliferate and expand in vitro in response to stimulation with nonpeptide antigens. PBL obtained from the macaques 4 to 6 weeks after BCG inoculation were stimulated in culture with partially purified Ag1 from M. fortuitum or BCG nonpeptide antigen. After 10 to 14 days of stimulation, cells were stained and analyzed by flow cytometry. Data shown are the means and standard errors of the means of values from four monkeys. (a) Data from SIVmac-infected macaques (left panel) and control macaques not infected with SIVmac (right panel). (b) The reduced capacity of Vγ2Vδ2+ PBL from SIVmac-BCG-coinfected monkeys to expand in vitro could be restored to some extent by coculture of these cells with CD4+ T cells but not the B-LCL cell line. P was <0.01 in comparisons of Vγ2Vδ2+ T-cell expansion between PBL cultures from SIVmac-infected monkeys and those from control animals; P was <0.05 in analyses of a difference in Vγ2Vδ2+ T-cell expansion between cultures with and without CD4+ T cells from SIV-negative monkeys.