FIG. 1.

Conserved mutations in replicons isolated from 26 independently selected replicon cell clones. A schematic drawing of the HCV NS proteins as present in the subgenomic replicons is shown. Regions encoding known enzymatic functions are shaded. Mutations conserved in the replicon population of single cell clones are given above. Numbers refer to the amino acid position in the complete polyprotein of the HCV Con-1 isolate. The frequency with which a given mutation was found in these cell clones is indicated in parentheses. Mutations identified as the only conserved alteration in at least one cell clone are underlined. A summary of these data is given in Table 1. Ins, insertion; del, deletion; prot., proteinase.