FIG. 6.

Comparable translation from the EMCV and the PV IRES in different Huh-7 passages. Replication-deficient RNAs containing a luciferase gene under the translational control of an EMVC (A) or a PV IRES (B) as indicated at the top of each panel were electroporated into different passages of Huh-7 cells. In vitro transcripts coding for β-galactosidase were cotransfected to normalize for transfection efficiency. Cells were harvested 2 h after electroporation, and the luciferase and β-galactosidase activities were determined. The given values are corrected for transfection efficiency. Passage numbers of the Huh-7 cells used for transfection are indicated under each column.