FIG. 10.

Sensitivity of UL51 protein to proteinase K. U251 cells expressing only the UL51w/t protein (A) and HSV-1-infected U251 cells (B) were treated with proteinase K in the absence (−) or presence (+) of 1% Triton X-100. All samples were then separated by SDS-PAGE and analyzed by Western blotting with the anti-UL51 rabbit polyclonal antibody and with mouse monoclonal antibodies against the Golgi-58K protein, GM130, γ-adaptin, β-COP, or TfR. Positions of molecular mass markers (in kilodaltons) are indicated on the left.