FIG. 11.

TIEM of HSV-1-infected U251 cells. Thin sections were prepared as described in Materials and Methods and incubated with preimmune rabbit serum (A) or the anti-UL51 rabbit polyclonal antibody (B) after treatment with 20% normal goat serum to block nonspecific antibody reactions. Samples were then incubated with anti-rabbit IgG-conjugated 10-nm-diameter gold particles. After extensive rinsing, sections were stained with uranyl acetate and lead citrate and examined with a Hitachi H7100 transmission electron microscope at 100 kV. Some of the enveloped capsids (arrows) were labeled. Labeling was also found in L-particle-like structures (large arrowheads) and along with membranous structures (small arrowheads). The inset shows an enveloped intracellular capsid labeled for UL51 proteins. Bars, 200 nm.