FIG. 4.

Effect of BFA on the distribution of UL51 protein and Golgi-58K protein. ST51 cells were pretreated with either AlF (g to i) or DOG-Az (j to l) for 10 min at 37°C and mock treated (a to c) or treated with BFA (5 μg/ml) (d to l) for 30 min at 37°C. Cells were then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. The cells were double stained with anti-UL51 rabbit polyclonal antibody and anti-Golgi-58K protein mouse monoclonal antibody and then reacted with anti-rabbit IgG-conjugated FITC and anti-mouse IgG-conjugated TRITC. Fluorescence images were obtained with the Bio-Rad MRC 1024 confocal imaging system.