FIG. 8.

Palmitoylation of UL51 protein. (A and B) Transfected U251 cells were labeled with [³⁵S]methionine (A) or [³H]palmitic acid (B) as described in Materials and Methods. Cell lysates were immunoprecipitated with anti-UL51 rabbit polyclonal antibody (lanes 1 and 3 to 6) or preimmune rabbit serum (lane 2), and the labeled proteins were mixed with the sample buffer (without β-mercaptoethanol). The samples were separated by SDS-PAGE and detected by autoradiography. (C) HSV-1-infected U251 cells were labeled with [³⁵S]methionine or [³H]palmitic acid. At 9 h p.i., cells were harvested and the lysates were immunoprecipitated and analyzed as described in Materials and Methods.