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The effect of various periods of incubation with IL-1β on hBD-2 expression by primary conjunctival epithelial cells. (A) RT-PCR products for GAPDH and hBD-2 from primary conjunctival cells (n = 1) treated with IL-1β for various lengths of incubation at a concentration of 10 ng/mL. M, size marker; C, media-treated controls. (B) The changes in hBD-2 protein secretion in this experiment. Similar results were obtained with the cell lines (n = 2).
