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Effects of soluble recombinant HveA or nectin-1 on viral entry into primary rat neurons. The panels shown depict representative neuronal cultures photographed using phase contrast (A, C, and E) or epifluorescence (B, D, and F) 24 h after infection. (A and B) HSVEGFP4 alone; (C and D) HSVEGFP4 with soluble HveA [HveA(200t)]; (E and F) HSVEGFP4 preincubated with soluble nectin-1 [HveC(346t)]. Images are shown at ×600 magnification.
