FIG. 2.

Effect of soluble recombinant HveA or nectin-1 on viral entry into primary rat neurons, mouse neurons, and primary rat fibroblasts. (A) Virus was incubated with soluble receptor before being added to cells. An MOI of 100 PFU per cell was used for rat and mouse sensory neurons. An MOI of 10 PFU per cell was used for rat fibroblasts. Each bar represents the average percentage of GFP-positive cells per culture. Error bars indicate standard error of the mean (n = 4). (B) HSVEGFP4 virus was incubated with the indicated concentrations of recombinant HveA(200t) (open squares) or nectin-1(346t) (closed diamonds) prior to infection of rat sensory neurons. Each point represents the average percentage of GFP-positive cells per culture.