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Detection of HveA and nectin-1 proteins by immunoblotting. For immunoblot analysis, 30 μg of protein from whole-cell lysates and 2 ng of soluble recombinant HveA(200t) or 10 ng of nectin-1(346t) were separated by electrophoresis and transferred to membranes for immunodetection. HveA was detected using rabbit anti-HveA serum (R140) (A), and nectin-1 was detected using rabbit anti-nectin-1 serum (R166) (B).
