Figure 2.

Biochemical analysis of FL and Δ14 CD31-EGFPs in transfected endothelioma cells. Untreated (-) and pervanadate treated (+) FL CD31-EGFP and Δ14 CD31-EGFP transfected tEnd.1 cells (denoted by “FL” and “Δ 14” respectively; “WT” indicates wild-type tEnd.1 cells) were lysed in lysis buffer. Sequential immunoprecipitations (IPs) were performed using anti-EGFP followed by anti-PECAM-1. Following SDS-PAGE and western blotting, blots were probed with anti-PTP SHP-2. The 68 kDa PTP SHP-2 coimmunoprecipitated with FL CD31-EGFP (by anti-EGFP in FL cells) and endogenous PECAM-1 (by anti-PECAM-1 IPs in all three cell lines) but not with Δ14 CD31-EGFP (by anti-EGFP in Δ14 cells).