Localization of cell adhesion molecules on
cocultured tEnd.1 and bEnd. PECAM-1.2neo cells. Immunofluorescence
staining of the cell adhesion molecules JAM (A), ICAM-2 (B), and
PECAM-1 (C) on cocultured tEnd.1 and bEnd. PECAM-1.2neo cells.
(D) represents a light image of (C) showing the prescence of bEnd.
PECAM-1.2neo cells contacting tEnd.1 cells. The JAM and ICAM-2 controls
showed the expected junctional and diffusive expression respectively.
PECAM-1 localized to junctions formed by PECAM-1 expressing cells (C,
left side) but not to junctions formed by PECAM-1 expressing and
deficient cells (C, arrows). Bars, 15 μm.