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Surface distribution of FL and Δ14 CD31-EGFPs on bEnd. PECAM-1.2neo cells. Fluorescence microscopy showing the surface distribution of FL CD31-EGFP (A) and Δ14 CD31-EGFP (B) on transiently transfected bEnd. PECAM-1.2neo cells. Both PECAM-1 splice variants preferentially localized to the endothelial cell junctions of transfected cells (arrows), but not to borders formed by PECAM-1 expressing and deficient cells (confluent cell monolayer). Bar, 15 μm.
