Table 1.

Drosophila melanogaster stocks used in this study

Stock	Genotype	Source/Reference
1	pnutrN498/CyO; rya	Neufeld and Rubin, 1994
2	w; P[mini w+, FRT]2R-G13, L/CyO	Chou and Perrimon, 1996b
3	w; P[mini w+, FRT]2R-G13, pnutrN498/CyO	1 × 2
4	w, P[ry+, HS-FLP]1; Adv1/CyO	Chou and Perrimon, 1996b
5	w, P[ry+, HS-FLP]1; P[mini w+, FRT]2R-G13, pnutrN498/CyO	3 × 4
6	P[mini w+, FRT]2R-G13, P[mini w+, ovoD1-18]2R1, P[mini w+, ovoD1-18]2R2/Dp(?;2), bwD, S1, Sp1, Ms(2)M/CyO	Chou and Perrimon, 1996b
7	w, P[ry+, HS-FLP]1/+; P[mini w+, FRT]2R-G13, pnutrN498/P[mini-w+, FRT]2R-G13, P[mini w+, ovoD1-18]2R1, P[mini w+, ovoD1-18]2R2	5 × 6
8	w; pnutxp/CyO; P[w+, HS-pnut+]c	Neufeld and Rubin, 1994
9	w; pnutxp; P[w+, HS-pnut+]d	8 × 8
10	w; P[w+, pnut+], ee	Neufeld and Rubin, 1994
11	w; Noc[Sco]/CyO	b
12	w; Noc[Sco]/CyO; P[w+, pnut+]	10 × 11
13	w; pnutrN498; P[w+, pnut+]	1 × 12
14	w; pnutxp; P[w+, pnut+]	8 × 12

^a pnut^rN498 is P[lacZ, ry⁺]^rN498; it carries the P[lacZ, ry⁺] element inserted at nucleotide 75 of the pnut coding region (Neufeld and Rubin, 1994). This lethal mutation is recessive to the wild-type pnut⁺ allele carried on the CyO balancer chromosome. 

^b This stock was obtained from the Bloomington Stock Center (Bloomington, IN). 

^c pnut^xp was generated by excision of the P element of the pnut^rN498 allele, leaving a small deletion that removes most of the pnut coding region (Neufeld and Rubin, 1994). P[w⁺, HS-pnut⁺] is a transgene carrying a wild-type pnut⁺ coding region under the control of the hsp70 promoter (Neufeld and Rubin, 1994). 

^d Mature adults of this genotype were produced by intermittently inducing transcription from the HS-pnut⁺ transgene by a 2-h heat shock (at 37°C) once per day. 

^e P[w⁺, pnut⁺] is a transgene inserted into chromosome 3; it contains 10 kb of genomic sequence, including the full pnut⁺ gene and flanking sequences.