Week 1:	Isolate plasmids from E. coli.
Week 2:	Digest the plasmids with restriction endonucleases; transform yeast cells with the plasmid DNA.
Week 3:	Analyze restriction digests by gel electrophoresis; set up Southern blots; plate transformants on appropriate media.
Week 4:	Complete Southern blots; class discussion/data analysis (Southern blots, restriction maps, phenotypic analysis of yeast transformants).