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. 2000 Sep;11(9):3137–3153. doi: 10.1091/mbc.11.9.3137

Figure 2.

Figure 2

Distribution of Syn7 and Syn13 in WIF-B cells by confocal microscopy. Polarized WIF-B cells were fixed and permeabilized and then double-labeled with antibodies to Syn7 and lgp120 (A), Syn13 and lgp120 (B), or Syn13 and TfR (C). Mouse mAbs were used to label Syn7 (A), lgp120 (B), and TfR (C) with rabbit polyclonal antibodies labeling lgp120 (A) and Syn13 (B and C). FITC-conjugated antibodies to mouse IgG (A, Syn7; B, lgp120; C, TfR) and Cy3-conjugated antibodies to rabbit IgG (A, lgp120; B and C, Syn13) were used as secondary antibodies. Optical sections in A and B are through the middle of the cells, whereas the section in C is slightly closer to the substratum to show more peripheral endosomes. Arrows point to structures that are positive for two markers, and arrowheads point to structures that are positive for only one marker. Note the good coincidence of Syn7 with lgp120-positive structures, i.e., lysosomes (A), and of Syn13 with TfR, i.e., early endosomes (C), whereas little overlap is seen between Syn13 and lgp120 (B). BC, bile canalicular-like space; N, nucleus. Bar, 10 μm.