Figure 8.

RGS4 inhibits secretion of placental alkaline phosphatase in HEK293T cells. Cells were transfected with plasmids encoding SEAP cytomegalovirus–β-galactosidase and a control vector or various HA–RGS4 plasmids. Forty-eight hours after transfection, medium was washed out and replaced with fresh medium for 4 h. Alkaline phosphatase was measured from the supernatant and cell lysate as luciferase activity and divided by β-galactosidase values to obtain normalized SEAP levels. The bar graph represents means ± SEM of two independent experiments (assayed in triplicate) expressed as a percentage of control vector alone (ratios of SEAP in supernatant and the sum of alkaline phosphatase in supernatant and cell lysate).