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. 2003 Mar 1;31(5):1565–1570. doi: 10.1093/nar/gkg227

Figure 3.

Figure 3

Primer extension analysis of the His3 promoter-bearing LexA operators. Primers from His3 and Pgk1 (internal control) were used to probe the transcriptional activities from His3 and Pgk1 promoters, respectively. Primer extension products are labeled. Major and minor transcriptional initiation sites (+1, +12 and +22) of the His3 gene are also labeled. Lanes 1, 2 and 3 show primer extension products from a vector (stem with an inactive loop), LexA-MS2-bound RNA activator no. 7, and LexA-Gal11, respectively.