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. 2003 Mar 1;31(5):1407–1415. doi: 10.1093/nar/gkg228

Figure 2.

Figure 2

Effect of plasmid copy number on the stability of cloned mouse mtDNA. (A) Stability of the mouse mtDNA in a low copy number E.coli strain. Plasmid pMusMtTN-ND5 was transformed into DH5α λatt::pirwt and was re-isolated after culturing in LB medium containing Cm (12.5 µg/ml) at 30 or 37°C for regular time intervals (24–72 h). Restriction patterns after SalI and SmaI digestion were compared on a 1% agarose gel. The top arrow points to the 16.3 kb mouse mtDNA and the lower to the band of the 1.5 kb γ-ori+CmR transposon. Lane M, λ DNA HindIII digest. (B) Stability of the mouse mtDNA in a high copy number E.coli strain. Mouse mtDNA clones were transformed into DH5α λatt::pir116 and were re-isolated from the recombinant E.coli after culturing in LB medium containing Cm at 37°C for 20 h. Restriction patterns after SalI digestion were compared on a 1% agarose gel. Lane M, 1 kb plus DNA ladder (Life Technologies); lanes 1–10, DNAs from DH5α λatt::pir116 strain; lane C, DNA from DH5α λatt::pirwt strain.