Figure 4.

Effect of adenosine deaminase on MGC formation. The fusion index was determined as detailed in Figure 3. P2X₇ hyper and P2X₇ hypo J774 cells were treated with hexokinase (HEXO) or apyrase (APY) and processed as described in Figure 2. FSDCs were incubated in the presence of PHA (10 μg/ml) or apyrase (0.4 U/ml) for 2 d before determination of fused cells. Adenosine deaminase was added at a concentration of 2 U/ml. C, control. Data are average ± SD of six determinations.