Figure 5.

MGC formation requires P2X₇ receptor expression on all cells participating to fusion. P2X₇ hyper and P2X₇ hypo FSDCs were allowed to pinocytose lucifer yellow (1 mg/ml) or Texas Red (0.4 mg/ml), respectively, for 3 h. At the end of this incubation time, the two batches were mixed in the well of a 24-well culture dish, and further incubated for 3 days. (A) Phase. (B) Rhodamine filter. (C) Fluorescein filter.