Figure 7.

Caspase 3 activation during cell fusion. Cells were seeded in 24-well culture plates and incubated either in culture medium alone (C) or in culture medium supplemented with ConA (10 μg/ml) for human macrophages, PHA (10 μg/ml) for FSDCs, and hexokinase (HEXO) for J774 macrophages. In some experiments, oATP (300 μM) or KN-62 (50 nM) also were added. After 2 d, when MGC formation was maximal, the monolayers cells were rinsed several times, lysed in lysis buffer provided with the EnzCheck kit, and assayed for caspase 3 activation. Data are average ± SD of six determinations.