Figure 6.

BIAcore SPR sensorgrams for the interaction of compounds 1 and 3 with the alternating AT and GC sequence DNA hairpins in HBS buffer at 25°C. Both compounds bind strongly to the AT sequence and reach saturation of the DNA in the concentration range of this experiment, 1–500 nM compound. In the same concentration range, no binding of compound 1 to the GC sequence is observed while significant binding of compound 3 can be detected. Fitting of results from these and additional experiments in the steady-state region provided data for determination of compound binding constants, as described in the Materials and Methods, and these are collected in Table 1.