Table 1.
Some neuronal morphology defects in sax-1 and sax-2 mutants are temperature sensitive
| ASEa
|
ASJb
|
AWCc
|
||||
|---|---|---|---|---|---|---|
| 25°C | 20°C | 25°C | 20°C | 25°C | 20°C | |
| Wild type | 2 (n = 110) | 1 (n = 114) | 4 (n = 154) | 0 (n = 203) | 0 (n = 136) | 2 (n = 118) |
| sax-1(ky211) | 84 (n = 102)d | 43 (n = 106) | 62 (n = 180)d | 27 (n = 241) | 92 (n = 129)d | 68 (n = 287) |
| sax-1(ky491) | 86 (n = 231)d | 64 (n = 110) | 59 (n = 286)d | 13 (n = 214) | 79 (n = 179) | 86 (n = 332) |
| sax-2(ky216) | 85 (n = 150) | 89 (n = 131) | 80 (n = 246)d | 28 (n = 167) | 97 (n = 150) | 94 (n = 185) |
Phenotypes were scored in adult animals raised at 25 or 20°C with the GFP fusion genes gcy-5∷gfp (ASE), tax-2Δ∷gfp (ASJ), and str-2∷gfp (AWC). Animals with cell bodies greater than or equal to twice the normal size or with ectopic neurites longer than the diameter of the cell body were scored as defective. n, number of animals scored.
sax-1 and sax-2 mutants exhibited predominantly cell shape defects in ASE and few ectopic neurites. For example, sax-1(ky491) at 25°C had 75% penetrant ASE cell shape defects and 11% penetrant ASE ectopic neurites (n = 231 animals).
sax-1 and sax-2 mutants exhibited exclusively ectopic neurite defects in ASJ and no cell shape defects.
sax-1 and sax-2 mutants exhibited predominantly cell shape defects in AWC and few ectopic neurites. For example, sax-1(ky491) at 25°C had 68% penetrant AWC cell shape defects and 12% penetrant AWC ectopic neurites (n = 179 animals).
These values differ between 25 and 20°C at p < 0.001.