Figure 6.

Morphological differentiation of pDuH-transfected cells mimics that of GAP-43 deficient PC12 cells. The GAP-43 deficient PC12 cell clone N36 (Tsai et al., 1997) was cultured under the same conditions as those described in Figure 5. After 96 h, cells were fixed and neurite outgrowth was visualized with Coomassie blue staining. Photographs were taken at 400X magnification. Quantitative analysis of cell morphology is shown in Table 2.