Table 1.

Correlation between HuD levels and GAP-43 mRNA half-life and protein expression in PC12 cells

PC12 Cells	HuD levelsa (% control)	mRNA Half-lifeb (T1/2 h)	% Change in T1/2c	GAP-43d (% control)
pDuH antisense transfected cells	30	3.4  ± 0.4	60	30
pcDNA3 transfected cells (vector)	100	5.1  ± 0.3	110	100
Untransfected PC12 cells (control)	100	5.0  ± 0.4	100	100
pcHuD transfected cells	300	9.0  ± 0.4	200	200

^a Relative levels of endogenous and exogenous HuD protein measured by Western blot analysis. 

^b GAP-43 mRNA half-life (T_1/2, mean ± S.E.M.) was measured as described in MATERIALS AND METHODS. 

^c Percent changes were calculated relative to control untransfected cells. 

^d GAP-43 protein levels were measured by Western blot analysis and were expressed relative to control cells.