Figure 2.

Necessity of rst2 for the transcription of ste11. Expression of ste11 was examined in the rst2⁺ (JY333) and rst2Δ (JX233) strains by Northern blot analysis. Total RNA was prepared from wild-type cells (lanes 1 and 2) and rst2Δ cells (lanes 3 and 4), either growing vegetatively (N+; lanes 1 and 3) or being starved for nitrogen (N−; lanes 2 and 4). rRNA stained with ethidium bromide is shown in the lower panel as loading controls. The relative intensity of ste11 transcription in each lane was calculated as described in MATERIALS AND METHODS and is presented under the top panel.