Figure 4.

The ste11 promoter region. (A) Scheme and nucleotide sequence of the promoter region of the ste11 gene. The SphI–BamHI segment, shown by a bold line, represents the 1.4-kb region newly sequenced in this study. Two TR boxes therein are indicated by open ovals. The open square represents UASst, which encloses a STRE-like element core 1. The same six-base motif in the upstream is denoted as core 2. The major transcription start site, which is assigned as position +1 for the nucleotide numbering, is indicated by the asterisk. Two hatched ovals in the downstream represent incomplete TR boxes noted previously. UASst, core 2, TR1, and TR2 are underscored by straight lines in the nucleotide sequence, whereas the incomplete TR boxes are underscored by dashed lines. Restriction sites indicated are B, BamHI; EV, EcoRV; N, NdeI, and Sp, SphI. (B) Assignment of the major transcription start site for ste11. The 5′ ends of the ste11 transcripts were determined by primer extension analysis. Total RNA prepared from cells either starved for nitrogen (lane 1) or growing logarithmically (lane 2) was used as the template. A single major start site (closed arrowhead) and an adjacent minor start site (open arrowhead) were detected.