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Kinetic constants for HsEH substrate hydrolysis
| Kinetic parameters | threo-PHO | para-NPP |
|---|---|---|
| Km, μM | 20.9 ± 0.30 | 1,600 ± 140 |
| VMax, nmol⋅min−1⋅mg−1 | 338 ± 12 | 57.6 ± 2.5 |
| kcat, s−1 | 0.35 ± 0.01 | 0.060 ± 0.003 |
| kcat/Km, M−1⋅s−1 | 16,700 ± 22 | 43.4 ± 0.47 |
| Hill coefficient | 1.9 ± 0.1 | 1.0 ± 0.0 |
