13C NMR signals of 6 obtained from
[1,3,4-13C3]5 (A) and
[2,2′-13C2]5
(B). Assay mixtures containing 50 mM Tris⋅HCl (pH 8.0),
0.5 mM deazaflavin, 100 μM 13C-labeled
5, and recombinant MalE/IspG fusion protein were
irradiated as described in Experimental Procedures.
*, A signal from an impurity.